Highly sensitive and accurate profiling of carotenoids by supercritical fluid chromatography coupled with mass spectrometry

被引:63
|
作者
Matsubara, Atsuki [2 ]
Bamba, Takeshi [1 ]
Ishida, Hiroki [2 ]
Fukusaki, Eiichiro [1 ]
Hirata, Kazumasa [2 ]
机构
[1] Osaka Univ, Grad Sch Engn, Dept Biotechnol, Suita, Osaka 5650871, Japan
[2] Osaka Univ, Grad Sch Pharmaceut Sci, Dept Appl Environm Biol, Suita, Osaka 5650871, Japan
关键词
Carotenoid; Mass spectrometry; Monolithic column; Supercritical fluid chromatography; SOL-GEL SYSTEM; PHASE-SEPARATION; STATIONARY PHASES; POLYACRYLIC-ACID; COLUMNS; LIPIDS;
D O I
10.1002/jssc.200800699
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We attempted to establish a high-speed and high-resolution profiling method for a carotenoid mixture as a highly selective and highly sensitive detection method; the analysis was carried out by supercritical fluid chromatography (SFC) coupled with mass spectrometry (MS). When an octadecyl-bonded silica (ODS) particle-packed column was used for separation, seven carotenoids including structural isomers were successfully separated within 15 min. This result indicated not only improved separation but also improved throughput compared to the separation and throughput in RP-HPLC. The use of a monolithic ODS column resulted in additional improvement in both the resolution and the throughput; the analysis time was reduced to 4 min by increasing the flow rate. Furthermore, carotenoids in biological samples containing the complex matrices were separated effectively by using several monolithic columns whose back pressure was very low. The mass spectrometer allowed us to perform a more sensitive analysis than UV detection; the detection limit of each carotenoid was 50 pg or below. This is the first report of carotenoid analysis carried out by SFC-MS. The profiling method developed in this study will be a powerful tool for carrying out accurate profiling of biological samples.
引用
收藏
页码:1459 / 1464
页数:6
相关论文
共 50 条
  • [1] Highly sensitive and rapid profiling method for carotenoids and their epoxidized products using supercritical fluid chromatography coupled with electrospray ionization-triple quadrupole mass spectrometry
    Matsubara, Atsuki
    Uchikata, Takato
    Shinohara, Masakazu
    Nishiumi, Shin
    Yoshida, Masaru
    Fukusaki, Eiichiro
    Bamba, Takeshi
    [J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2012, 113 (06) : 782 - 787
  • [2] Rapid profiling of cytokinins using supercritical fluid chromatography coupled with tandem mass spectrometry
    Petrik, Ivan
    Pencik, Ales
    Styskala, Jakub
    Tranova, Lenka
    Amakorova, Petra
    Strnad, Miroslav
    Novak, Ondrej
    [J]. ANALYTICA CHIMICA ACTA, 2024, 1285
  • [3] Supercritical fluid chromatography coupled to mass spectrometry for lipidomics
    Chollet, Celine
    Boutet-Mercey, Stephanie
    Laboureur, Laurent
    Rincon, Carlos
    Mejean, Marie
    Jouhet, Juliette
    Fenaille, Francois
    Colsch, Benoit
    Touboul, David
    [J]. JOURNAL OF MASS SPECTROMETRY, 2019, 54 (10): : 791 - 801
  • [4] Metabolic profiling of lipids by supercritical fluid chromatography/mass spectrometry
    Bamba, Takeshi
    Lee, Jae Won
    Matsubara, Atsuki
    Fukusaki, Eiichiro
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2012, 1250 : 212 - 219
  • [5] Supercritical fluid chromatography coupled to mass spectrometry - A metabolomics perspective
    Shulaev, Vladimir
    Isaac, Giorgis
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2018, 1092 : 499 - 505
  • [6] SUPERCRITICAL FLUID CHROMATOGRAPHY-MASS SPECTROMETRY OF CAROTENOIDS AND OTHER POLAR LIPIDS
    FREW, NM
    JOHNSON, CG
    [J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1987, 193 : 19 - AGFD
  • [7] Supercritical fluid chromatography/Orbitrap mass spectrometry based lipidomics platform coupled with automated lipid identification software for accurate lipid profiling
    Yamada, Takayuki
    Uchikata, Takato
    Sakamoto, Shigeru
    Yokoi, Yasuto
    Nishiumi, Shin
    Yoshida, Masaru
    Fukusaki, Eiichiro
    Bamba, Takeshi
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2013, 1301 : 237 - 242
  • [8] A highly sensitive method (supercritical fluid chromatography coupled with ion mobility mass spectrometry) for determination of multiple compounds in radix curcumae
    Ye, Li-Hong
    Dong, Xin
    Cao, Jun
    [J]. BIOMEDICAL CHROMATOGRAPHY, 2023, 37 (01)
  • [9] Application of supercritical fluid chromatography/mass spectrometry to lipid profiling of soybean
    Lee, Jae Won
    Uchikata, Takato
    Matsubara, Atsuki
    Nakamura, Takuji
    Fukusaki, Eiichiro
    Bamba, Takeshi
    [J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2012, 113 (02) : 262 - 268
  • [10] CAPILLARY SUPERCRITICAL FLUID CHROMATOGRAPHY AND SUPERCRITICAL FLUID CHROMATOGRAPHY-MASS SPECTROMETRY
    SMITH, RD
    WRIGHT, BW
    UDSETH, HR
    [J]. ACS SYMPOSIUM SERIES, 1986, 297 : 260 - 293