Carbonic anhydrase IX: A promising diagnostic and prognostic biomarker in breast carcinoma

被引:6
|
作者
Furjelova, Martina [1 ]
Kovalska, Maria [1 ]
Jurkova, Katarina [1 ,3 ]
Horacek, Jaroslav [2 ]
Carbolova, Tereza [4 ]
Adamkov, Marian [1 ]
机构
[1] Comenius Univ, Dept Histol & Embryol, Jessenius Fac Med, Martin 03601, Slovakia
[2] Univ Ostrava, Fac Med, Dept Pathol, Ostrava 70300, Czech Republic
[3] Comenius Univ, Dept Med Biochem, Jessenius Fac Med, Martin 03601, Slovakia
[4] Univ Ostrava, Fac Med, Dept Histol & Embryol, Ostrava 70300, Czech Republic
关键词
Breast cancer; Carbonic anhydrase IX; Immunohistochemistry; Tumor biomarker; TUMOR HYPOXIA; MN/CA IX; EXPRESSION; NECROSIS; THERAPY; CANCER; TISSUE; CELL; SURVIVAL; PATHWAY;
D O I
10.1016/j.acthis.2013.05.009
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We examined the expression of carbonic anhydrase IX (CA IX) by immunohistochemical staining using monoclonal antibody M75 (Institute of Virology, Slovak Academy of Sciences, Bratislava) in a group of 38 fibroadenomas and 55 carcinomas of the breast. In each case, the intensity of staining, percentage of labeled cells and subcellular localization of CA IX were assessed. CA IX was detected in 11/38 fibroadenomas (28.9%). Weak cytoplasmic positivity was dominant in these positive cases. Immunohistochemical analysis of 55 carcinomas showed CA IX expression in 34 cases (61.8%). Membrane staining alone was observed in 27/55 carcinomas (49.1%), while cytoplasmic positivity was found in 4/55 cases (7.3%). Combined membrane and cytoplasmic immunostaining of CA IX was detected in 3/55 carcinomas (5.4%). The intensity of immunoreactivity varied from weak to strong. Under 50% of reactive cells were found in 9/38 fibroadenomas (23.6%) and in 29/55 carcinomas (52.7%). More than 50% of reactive cells were found in 2/38 fibroadenomas (5.3%) and in 5/55 carcinomas (9.1%). Statistical analysis confirmed significant differences in the subcellular localization, intensity of immunoreactivity and percentage of labeled cells in fibroadenomas and carcinomas (p < 0.05). Our results confirmed the hypothesis that expression of CA IX may represent a valuable tumor biomarker as well as a promising diagnostic and prognostic parameter in breast cancer. (C) 2013 Elsevier GmbH. All rights reserved.
引用
收藏
页码:89 / 93
页数:5
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