Profiles of Pregnancy-Associated Glycoprotein (Pag) Concentrations during Gestation in Swamp Buffalo

The pregnancy-associated glycoproteins (PAG) are synthesized in the mono-and binucleate cells of the ruminant's trophectoderm. It was used as marker not only for early diagnosis pregnancy but also for determination stage of pregnancy. In this report, we present the results of establishing the profiles PAG concentration during time of gestation in swamp buffalo. A total 236 samples including maternal plasma, fetal plasma, allantoid and amniotic fluids were collected from buffalo slaughtered. The age of fetus were estimated from 6 - 48 weeks of pregnancy by base on crown-rum length measurement method. The PAG concentration was detected by RIA using antibody anti-buffalo PAG. The results shown that the mean of PAG concentration were variances in differences samples kind at the same animal. The PAG concentration there is a linear correlation between PAG concentration in plasma samples and time of pregnancy (Y = Ax(2) + Bx + C) whereas the A, B and C are depend on the maternal or fetus. In conclusion: the PAG concentrations in swamp buffalo are strictly correlated with the time of gestation.