PI3K/AKT Mediated p53 Down-Regulation Participates in CpG DNA Inhibition of Spontaneous B Cell Apoptosis

被引：10

作者：

Zhou, Yongxin ^{[2
]}

Zhen, Huiling ^{[1
]}

Mei, Yunqing ^{[2
]}

Wang, Yongwu ^{[2
]}

Feng, Jing ^{[2
]}

Xu, Shuchang ^{[1
]}

Fu, Xiaoying ^{[1
]}

机构：

[1] Tongji Univ, Tongji Hosp, Dept Gastroenterol, Shanghai 200065, Peoples R China

[2] Tongji Univ, Tongji Hosp, Dept Cardiovasc Thorac Surg, Shanghai 200065, Peoples R China

来源：

CELLULAR & MOLECULAR IMMUNOLOGY | 2009年 / 6卷 / 03期

关键词：

CpG DNA; PI3K; AKT; p53; apoptosis; SIGNALING PATHWAY; PROTEIN-KINASE; IMMUNOSTIMULATORY DNA; CASPASE ACTIVATION; AKT; DEATH; PHOSPHORYLATION; UBIQUITINATION; SURVIVAL; IMMUNITY;

D O I：

10.1038/cmi.2009.24

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The unmethylated CpG DNA can prevent spontaneous apoptosis of B cells. However, the precise mechanisms by which CpG DNA blocks apoptosis remain unclear. In this study, we showed B cell apoptosis was significantly inhibited by addition of CpG DNA. Treatment of CpG DNA could reduce the expression of caspase 3, increase JAP and Bcl-xL expressions, and inhibit p53 protein expression which level was increased in B cell spontaneous apoptosis at 24 h. AKT kinase activity was increased with the incubation of CpG DNA. The wortmannin and Ly294002 could abrogate the protection of B cell from apoptosis by CpG DNA. The up-regulations of Bcl-xL and IAP by CpG DNA were not inhibited when blocking PI3K by specific inhibitor Ly294002, while the inhibition of p53 by CpG DNA could be blocked by Ly294002. These results demonstrated that the inhibition of spontaneous B cell apoptosis by CpG DNA was correlated to up-regulation of Bcl-xL, IAP and down-regulation of p53 and caspase 3. CpG DNA inhibition of p53 is mediated through PI3K/AKT signaling. Cellular & Molecular Immunology. 2009; 6(3):175-180.

引用

页码：175 / 180

页数：6

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