Expression of the IGF-1, IGFBP-3 and IGF-1 receptors in dental pulp stem cells and impacted third molars

被引:24
|
作者
Magnucki, Gabriel [1 ,2 ]
Schenk, Ulf [2 ]
Ahrens, Stefan [2 ]
Santos, Alexander Navarrete [3 ]
Gernhardt, Christian R. [1 ]
Schaller, Hans-Guenter [1 ]
Cuong Hoang-Vu [2 ]
机构
[1] Univ Halle Wittenberg, Fac Med, Univ Sch Dent Med, Dept Operat Dent & Periodontol, D-06097 Halle, Germany
[2] Univ Halle Wittenberg, Fac Med, Dept Gen Visceral & Vasc Surg, D-06097 Halle, Germany
[3] Univ Halle Wittenberg, Fac Med, Dept Cardiothorac Surg, D-06097 Halle, Germany
关键词
dental pulp stem cell; dentinogenesis; IGF-1; receptor; odontogenesis; GROWTH-FACTOR-I; HUMAN BONE-MARROW; BINDING-PROTEINS; TOOTH DEVELOPMENT; INSULIN; VITRO; DIFFERENTIATION; TEETH; INDUCTION; BIOLOGY;
D O I
10.2334/josnusd.55.319
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
IGF-1 regulates the metabolism of hard dental tissue through binding to the IGF-1 receptor on target cells. Furthermore, IGF-binding-protein-3 promotes the accessibility of IGF-1. The aim of this study was to investigate the expression of IGF-1, IGFBP-3 and IGF-1R in STRO-1-positive dental pulp stem cells (DPSCs) and fully impacted wisdom teeth in relation to tooth development. Third molars were surgically removed from 60 patients and classified into two groups: teeth showing ongoing development (group 1) and teeth that had completed root shaping (group 2). The transcript and protein levels of IGF-1, IGFBP-3 and IGF-1R were investigated using RT-PCR and immunohistochemistry. The expression of the same proteins was also analyzed in DPSCs. The teeth from group 1 showed significantly stronger expression of IGF-1 and IGF-1R. The major sources of all of the proteins investigated immunohistochemically in sections of wisdom teeth were odontoblasts, cementoblasts and cell colonies in the pulpal mesenchyme. These colonies were identified as stem cells in view of their positivity for STRO-1, and the cells were subsequently sorted by flow cytometry. These DPSCs demonstrated high levels of pluripotency markers and IGF-1 and IGF-1R. We conclude that members of the IGF-1 family are involved in the late stage of tooth development and the process of pulpal differentiation.
引用
收藏
页码:319 / 327
页数:9
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