A Mouse Model to Investigate the Role of Cancer-associated Fibroblasts in Tumor Growth

被引：4

作者：

Jelinek, David ^{[1
,2
]}

Zhang, Ellen Ran ^{[1
,2
,3
]}

Ambrus, Aaron ^{[1
,2
]}

Haley, Erin ^{[3
]}

Guinn, Emily ^{[1
,2
]}

Vo, Austin ^{[1
,2
]}

Le, Peter ^{[1
,2
]}

Kesaf, Ayse Elif ^{[1
,2
]}

Nguyen, Jennifer ^{[1
,2
]}

Guo, Lily ^{[1
,2
]}

Frederick, Destiny ^{[1
,2
]}

Sun, Zhengyang ^{[1
,2
]}

Guo, Natalie ^{[3
]}

Sevier, Parker ^{[1
,2
]}

Bilotta, Eric ^{[1
,2
]}

Atai, Kaiser ^{[1
,2
,4
]}

Voisin, Laurent ^{[1
,2
]}

Coller, Hilary A. ^{[1
,2
,4
]}

机构：

[1] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Biol Chem, Los Angeles, CA 90095 USA

[3] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA

[4] Univ Calif Los Angeles, Inst Mol Biol, Los Angeles, CA 90024 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2020年 / 166期

基金：

美国国家卫生研究院;

关键词：

CARCINOMA-ASSOCIATED FIBROBLASTS; CD8(+) T-CELLS; FIBROSIS; INDUCE; QUIESCENCE; EXPRESSION; MATRICES; DELETION; MICE;

D O I：

10.3791/61883

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cancer-associated fibroblasts (CAFs) can play an important role in tumor growth by creating a tumor-promoting microenvironment. Models to study the role of CAFs in the tumor microenvironment can be helpful for understanding the functional importance of fibroblasts, fibroblasts from different tissues, and specific genetic factors in fibroblasts. Mouse models are essential for understanding the contributors to tumor growth and progression in an in vivo context. Here, a protocol in which cancer cells are mixed with fibroblasts and introduced into mice to develop tumors is provided. Tumor sizes over time and final tumor weights are determined and compared among groups. The protocol described can provide more insight into the functional role of CAFs in tumor growth and progression.

引用

页数：14

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