Isolation and characterization of 24 microsatellite loci in Paphiopedilum rothschildianum, an endangered slipper orchid

被引:2
|
作者
Rodrigues, K. F. [1 ]
Kumar, S. V. [1 ]
机构
[1] Univ Malaysia Sabah, Biotechnol Res Inst, Kota Kinabalu 88999, Saga, Malaysia
关键词
Paphiopedilum rothschildianum; Microsatellite; 5 ' anchored PCR; Heterozygosity; CITES;
D O I
10.1007/s10592-008-9533-1
中图分类号
X176 [生物多样性保护];
学科分类号
090705 ;
摘要
This paper describes the characterization of 24 polymorphic microsatellite loci in Paphiopedilum rothschildianum, an endangered slipper orchid endemic to Sabah, Malaysia. A total of 36 clones containing microsatellite repeat motifs yielded 41 dinucleotide, 20 tetranucleotide and 30 cryptic simple repeat sequences. Thirty microsatellite loci were randomly selected to characterize the population, of which 24 were found to be polymorphic. The expected heterozygosity ranged from 0.0813 to 0.7279 (mean 0.4533) and the observed heterozygosity ranged from 0 to 1 (mean 0.3800). The results indicated that there was limited migration between the three subpopulations and a significant degree of genetic differentiation (F-ST = 0.5098) between populations implying that gene flow has been disrupted as a result of habitat fragmentation. The molecular markers have a potential application in population management, CITES enforcement and the forensic detection of specimens in international trade.
引用
收藏
页码:127 / 130
页数:4
相关论文
共 50 条
  • [1] Isolation and characterization of 24 microsatellite loci in Paphiopedilum rothschildianum, an endangered slipper orchid
    K. F. Rodrigues
    S. V. Kumar
    [J]. Conservation Genetics, 2009, 10 : 127 - 130
  • [2] In vitro multiplication of the rare and endangered slipper orchid, Paphiopedilum rothschildianum (Orchidaceae)
    Ng, Chyuam-Yih
    Saleh, Norihan Mohd.
    Zaman, Faridah Qamaruz
    [J]. AFRICAN JOURNAL OF BIOTECHNOLOGY, 2010, 9 (14): : 2062 - 2068
  • [3] ISOLATION AND CHARACTERIZATION OF NOVEL MICROSATELLITE LOCI FOR THE ENDANGERED ORCHID CYPRIPEDIUM JAPONICUM (ORCHIDACEAE)
    Yamashita, Yumi
    Izuno, Ayako
    Isagi, Yuji
    Kurosawa, Takahide
    Kaneko, Shingo
    [J]. APPLICATIONS IN PLANT SCIENCES, 2016, 4 (02):
  • [4] Asymbiotic germination and seed storage of Paphiopedilum insigne, an endangered lady's slipper orchid
    Diengdoh, Reema Vareen
    Kumaria, Suman
    Tandon, Pramod
    Das, Meera Chettri
    [J]. SOUTH AFRICAN JOURNAL OF BOTANY, 2017, 112 : 215 - 224
  • [5] Isolation and characterization of microsatellite loci in the Brazilian orchid Epidendrum fulgens
    F. Pinheiro
    M. O. Santos
    F. Barros
    D. Meyer
    A. Salatino
    A. P. Souza
    S. Cozzolino
    [J]. Conservation Genetics, 2008, 9 : 1661 - 1663
  • [6] Isolation and characterization of microsatellite loci in the Brazilian orchid Epidendrum fulgens
    Pinheiro, F.
    Santos, M. O.
    Barros, F.
    Meyer, D.
    Salatino, A.
    Souza, A. P.
    Cozzolino, S.
    [J]. CONSERVATION GENETICS, 2008, 9 (06) : 1661 - 1663
  • [7] Characterization of microsatellite loci in the endangered grand spider orchid Caladenia huegelii (Orchidaceae)
    Swarts, Nigel D.
    Sinclair, Elizabeth A.
    Dixon, Kingsley W.
    [J]. MOLECULAR ECOLOGY NOTES, 2007, 7 (06): : 1141 - 1143
  • [8] Isolation and characterization of microsatellite loci in endangered Cycas changjiangensis (Cycadaceae)
    Li, Ling
    Wang, Zheng-Feng
    Jian, Shu-Guang
    Zhu, Peng
    Zhang, Min
    Ye, Wan-Hui
    Ren, Hai
    [J]. CONSERVATION GENETICS, 2009, 10 (03) : 793 - 795
  • [9] Isolation and characterization of microsatellite loci in endangered Cycas changjiangensis (Cycadaceae)
    Ling Li
    Zheng-Feng Wang
    Shu-Guang Jian
    Peng Zhu
    Min Zhang
    Wan-Hui Ye
    Hai Ren
    [J]. Conservation Genetics, 2009, 10 : 793 - 795
  • [10] Characterization of the complete chloroplast genome of Paphiopedilum micranthum, an Endangered orchid in China
    Chen, Xian-De
    Peng, Dong-Hui
    Lan, Si-Ren
    Chen, Jun
    Fu, Wen-Qun
    [J]. MITOCHONDRIAL DNA PART B-RESOURCES, 2020, 5 (01): : 115 - 116