miR-140-5p suppresses BMP2-mediated osteogenesis in undifferentiated human mesenchymal stem cells

被引：147

作者：

Hwang, Supyong ^{[1
]}

Park, Seul-Ki ^{[1
,2
]}

Lee, Ha Yeon ^{[1
]}

Kim, Seong Who ^{[3
]}

Lee, Jung Shin ^{[4
,5
]}

Choi, Eun Kyung ^{[4
,6
,7
]}

You, Dalsan ^{[8
]}

Kim, Choung-Soo ^{[8
]}

Suh, Nayoung ^{[1
,2
]}

机构：

[1] Asan Med Ctr, Asan Inst Life Sci, Seoul 138736, South Korea

[2] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Med, Seoul 138736, South Korea

[3] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Biochem & Mol Biol, Seoul 138736, South Korea

[4] Univ Ulsan, Coll Med, Asan Med Ctr, Inst Innovat Canc Res, Seoul 138736, South Korea

[5] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Internal Med, Seoul 138736, South Korea

[6] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Radiat Oncol, Seoul 138736, South Korea

[7] Univ Ulsan, Coll Med, Asan Med Ctr, Ctr Dev & Commercializat Anticanc Therapeut, Seoul 138736, South Korea

[8] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Urol, Seoul 138736, South Korea

来源：

FEBS LETTERS | 2014年 / 588卷 / 17期

基金：

新加坡国家研究基金会;

关键词：

Human mesenchymal stem cells; miR-140-5p; Bone morphogenic protein 2; Differentiation; Osteogenic lineage commitment; HUMAN ADIPOSE-TISSUE; OSTEOBLAST DIFFERENTIATION; MICRORNA SIGNATURE; BONE-FORMATION; TARGETS; PROLIFERATION; BIOGENESIS; LINEAGE; MARROW; BETA;

D O I：

10.1016/j.febslet.2014.05.048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human mesenchymal stem cells (hMSCs) have self-renewal and differentiation capabilities but the regulatory mechanisms of MSC fate determination remain poorly understood. Here, we aimed to identify microRNAs enriched in hMSCs that modulate differentiation commitments. Microarray analysis revealed that miR-140-5p is commonly enriched in undifferentiated hMSCs from various tissue sources. Moreover, bioinformatic analysis and luciferase reporter assay validated that miR-140-5p directly represses bone morphogenic protein 2 (BMP2). Furthermore, blocking miR-140-5p in hMSCs increased the expression of BMP signaling components and critical regulators of osteogenic differentiation. We propose that miR-140-5p functionally inhibits osteogenic lineage commitment in undifferentiated hMSCs. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2957 / 2963

页数：7

共 50 条

[1] miR-140-5p suppresses BMP2-mediated osteogenesis in undifferentiated human mesenchymal stem cells
Suh, N.
Hwang, S.
Park, S. -K.
[J]. FEBS JOURNAL, 2014, 281 : 231 - 231
[2] MiR-140-5p suppresses the adipogenic differentiation of mesenchymal stem cells in rats with steroid-induced osteonecrosis of the femoral head
Lu, Shengjun
Qi, Baiwen
Liao, Quanming
Yu, Aixi
[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE, 2020, 13 (02): : 549 - 557
[3] Long non-coding RNA MEG3 inhibits adipogenesis and promotes osteogenesis of human adipose-derived mesenchymal stem cells via miR-140-5p
Li, Zheng
Jin, Chanyuan
Chen, Si
Zheng, Yunfei
Huang, Yiping
Jia, Lingfei
Ge, Wenshu
Zhou, Yongsheng
[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2017, 433 (1-2) : 51 - 60
[4] Long non-coding RNA MEG3 inhibits adipogenesis and promotes osteogenesis of human adipose-derived mesenchymal stem cells via miR-140-5p
Zheng Li
Chanyuan Jin
Si Chen
Yunfei Zheng
Yiping Huang
Lingfei Jia
Wenshu Ge
Yongsheng Zhou
[J]. Molecular and Cellular Biochemistry, 2017, 433 : 51 - 60
[5] LncRNA H19 promotes odontoblastic differentiation of human dental pulp stem cells by regulating miR-140-5p and BMP-2/FGF9
Jialin Zhong
Xinran Tu
Yuanyuan Kong
Liyang Guo
Baishun Li
Wenchao Zhong
Ying Cheng
Yiguo Jiang
Qianzhou Jiang
[J]. Stem Cell Research & Therapy, 11
[6] LncRNA H19 promotes odontoblastic differentiation of human dental pulp stem cells by regulating miR-140-5p and BMP-2/FGF9
Zhong, Jialin
Tu, Xinran
Kong, Yuanyuan
Guo, Liyang
Li, Baishun
Zhong, Wenchao
Cheng, Ying
Jiang, Yiguo
Jiang, Qianzhou
[J]. STEM CELL RESEARCH & THERAPY, 2020, 11 (01)
[7] Small Molecule–Mediated Enhanced Osteogenesis of Human Mesenchymal Stem Cells: a Probable Alternate for BMP-2
Pearlin Hameed
Dwaipayan Sen
Geetha Manivasagam
[J]. Regenerative Engineering and Translational Medicine, 2020, 6 : 407 - 418
[8] miR-140-5p protects cartilage progenitor/stem cells from fate changes in knee osteoarthritis
Chen, Yang
Huang, Hua
Zhong, Wen
Li, Lan
Lu, Yanrong
Si, Hai-bo
[J]. INTERNATIONAL IMMUNOPHARMACOLOGY, 2023, 114
[9] BMP-2-mediated regulation in osteogenesis of human mesenchymal stem cells under dexamethasone-free condition
不详
[J]. CALCIFIED TISSUE INTERNATIONAL, 2006, 78 : S52 - S53
[10] Small Molecule-Mediated Enhanced Osteogenesis of Human Mesenchymal Stem Cells: a Probable Alternate for BMP-2
Hameed, Pearlin
Sen, Dwaipayan
Manivasagam, Geetha
[J]. REGENERATIVE ENGINEERING AND TRANSLATIONAL MEDICINE, 2020, 6 (04) : 407 - 418

← 1 2 3 4 5 →