An improved protocol for the preparation of protoplasts from an established Arabidopsis thaliana cell suspension culture and infection with RNA of turnip yellow mosaic tymovirus:: a simple and reliable method

被引：50

作者：

Schirawski, J

Planchais, S

Haenni, AL

机构：

[1] Univ Paris 11, Inst Biotechnol Plantes, F-91405 Orsay, France

[2] State Univ Ghent VIB, Genet Lab, B-9000 Ghent, Belgium

[3] Inst Jacques Monod, F-75251 Paris, France

来源：

JOURNAL OF VIROLOGICAL METHODS | 2000年 / 86卷 / 01期

关键词：

Arabidopsis thaliana; protoplast; plant virus; turnip yellow mosaic tymovirus; transfection;

D O I：

10.1016/S0166-0934(99)00173-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An improved method for preparation of protoplasts of Arabidopsis thaliana cells grown in suspension culture is presented. This method is fast, reliable and can be used for the production of virtually an unlimited number of protoplasts at any time. These protoplasts can be transformed efficiently with RNA from turnip yellow mosaic tymovirus (TYMV) by polyethyleneglycol-mediated transfection. The simple transfection procedure has been optimized at various steps. Replication of TYMV can be monitored routinely by detection of the coat protein in as few as 2 x 10(4) infected protoplasts. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：85 / 94

页数：10