T-2 toxin-induced cytotoxicity and damage on TM3 Leydig cells

被引：42

作者：

Yuan, Zhihang ^{[1
]}

Matias, Froilan Bernard ^{[2
]}

Yi, Jin-e ^{[1
]}

Wu, Jing ^{[1
]}

机构：

[1] Hunan Agr Univ, Dept Clin Vet Med, Coll Vet Med, Changsha 410128, Hunan, Peoples R China

[2] Coll Vet Sci & Med, Dept Anim Management, Sci City Of Munoz 3120, Nueva Ecija, Philippines

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY | 2016年 / 181卷

基金：

中国国家自然科学基金;

关键词：

T-2; toxin; TM3 Leydig cells; Cytotoxicity; Oxidative stress; DNA damage; Apoptosis; OXIDATIVE STRESS; DNA-DAMAGE; LIPID-PEROXIDATION; DEOXYNIVALENOL; APOPTOSIS; EXPOSURE; STEROIDOGENESIS; MITOCHONDRIA; MYCOTOXINS; INDUCTION;

D O I：

10.1016/j.cbpc.2015.12.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

T-2 toxin is a highly toxic mycotoxin produced by various Fusarium species, mainly, Fusarium sporotrichoides, and has been reported to have toxic effects on reproductive system of adult male animals. This study investigated the dose-dependent cytotoxicity of T-2 toxin on reproductive cells using TM3 Leydig cells. Specifically, the cytotoxic effect of T-2 toxin was assessed by measuring cell viability; lactate dehydrogenase (LDH); malondialdehyde (MDA); antioxidant activity by measuring superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX), and DNA damage; and cell apoptosis. Results showed that T-2 toxin is highly cytotoxic on TM3 Leydig cells. However, Trolox-treated TM3 Leydig cells showed significantly reduced oxidative damage, DNA damage, and apoptosis induced by T-2 toxin. This study proves that T-2 toxin can damage the testes and thus affects the reproductive capacity of animals and humans. Furthermore, oxidative stress plays an important role in the cytotoxic effect of T-2 toxin. (C) 2015 Published by Elsevier Inc.

引用

页码：47 / 54

页数：8

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