Osteopontin in Seminal Plasma and Sperm Membrane of Dogs

被引：15
|
作者：
Souza, F. F. [1 ]
Chirinea, V. H. [2 ]
Martins, M. I. M. [3 ]
Lopes, M. D. [2 ]
机构：
[1] Univ Franca, Hosp Vet, BR-14404600 Franca, SP, Brazil
[2] UNESP, Dept Reprod Anim & Radiol Vet, FMVZ, Botucatu, SP, Brazil
[3] Univ Estadual Londrina, Dept Clin Vet, Londrina, Brazil
来源：
REPRODUCTION IN DOMESTIC ANIMALS | 2009年 / 44卷
关键词：
PROTEIN; IDENTIFICATION; TESTIS;
D O I：
10.1111/j.1439-0531.2009.01447.x
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The objective of this work was to describe the presence of osteopontin (OPN) in canine seminal plasma and sperm membranes. A pool of seminal plasma and sperm membrane extract from 30 dogs was used. Polyacrylamide electrophoresis gels were performed and the bands were transferred to nitrocellulose paper and Western blot was undertaken using an antibody anti-OPN. Two and 12 bands were marked in the seminal plasma (77.2 and 15.6 kDa) and sperm membrane extracts (70.6-26.6 kDa), respectively. However, from 12 marked bands in the sperm membrane extract, only three (46.4, 37.7 and 36.5 kDa) were strongly marked. We conclude that, seminal plasma and sperm membranes from dogs contain different isoforms of OPN; yet, further studies will be necessary to determine their function in this species.
引用
收藏
页码：283 / 286
页数：4
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