Modifying ligand specificity of gene regulatory proteins

被引：8

作者：

Doyle, DF

Mangelsdorf, DJ

Corey, DR ^{[1
]}

机构：

[1] Univ Texas, SW Med Ctr, Dept Pharmacol, Howard Hughes Med Inst, Dallas, TX 75235 USA

[2] Univ Texas, SW Med Ctr, Dept Biochem, Howard Hughes Med Inst, Dallas, TX 75235 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2000年 / 4卷 / 01期

关键词：

D O I：

10.1016/S1367-5931(99)00052-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear receptors contain a conserved hydrophobic ligand binding pocket that is particularly amenable to structure-based protein engineering. Thus, site-directed mutagenesis of the ligand binding pocket has resulted in the creation of nuclear receptors with novel ligand specificities. Such proteins are now being used to control gene expression in vivo in a ligand-dependent manner.

引用

页码：60 / 63

页数：4

共 50 条

[1] Affinity, Specificity, and Cooperativity of DNA Binding by Bacterial Gene Regulatory Proteins
Carey, Jannette
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022, 23 (01)
[2] Determination of DNA binding specificity of regulatory proteins.
Maillet, AL
Hamilton, DA
Nagel, WO
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1997, 213 : 194 - CHED
[3] Conformational Motion in Gene Regulatory Proteins
Svintradze, David V.
BIOPHYSICAL JOURNAL, 2014, 106 (02) : 652A - 652A
[4] GENE REGULATORY PROTEINS AND THEIR INTERACTION WITH DNA
KLUG, A
DNA: THE DOUBLE HELIX: PERSPECTIVE AND PROSPECTIVE AT FORTY YEARS, 1995, 758 : 143 - 160
[5] LIGAND-BINDING PROCESS AND REGULATORY PROPERTIES OF PROTEINS
CHUKNYISKI, P
KAVARJIKOV, V
ZLATANOV, I
BIOFIZIKA, 1976, 21 (06): : 1124 - 1125
[6] RAMPs (receptor activity modifying proteins) as determinants of GPCR ligand selectivity
Marshall, FH
Foord, SM
BRITISH JOURNAL OF PHARMACOLOGY, 2000, 131 : U82 - U82
[7] Engineered acetylcholine binding proteins to explore ligand recognition and specificity
Bobango, Janet
Wensel, Skylar
Hunt, James
Amerhamzeh, Jennifer
Estep, Katrina
Talley, Todd T.
BIOCHEMICAL PHARMACOLOGY, 2015, 97 (04) : 627 - 628
[8] LIGAND SPECIFICITY OF PHEROMONE-BINDING PROTEINS OF THE PROCESSIONARY MOTH
FEIXAS, J
PRESTWICH, GD
GUERRERO, A
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 234 (02): : 521 - 526
[9] Redox reactions of regulatory proteins: do kinetics promote specificity?
Danon, A
TRENDS IN BIOCHEMICAL SCIENCES, 2002, 27 (04) : 197 - 203
[10] Modifying signal peptides for respiratory gene therapy with secreted proteins
Hickmott, J. W.
Prasertsuk, P.
Bell, R. V.
Chan, M.
Griesenbach, U.
Alton, E. W. F. W.
HUMAN GENE THERAPY, 2021, 32 (19-20) : A39 - A40

← 1 2 3 4 5 →