Integrating nucleic acid sequence-based amplification and microlensing for high-sensitivity self-reporting detection

被引：4

作者：

Teng, Feiyue ^{[1
]}

Wu, Xinpei ^{[1
]}

Hong, Tao ^{[2
,3
]}

Munk, Gary B. ^{[3
,4
]}

Libera, Matthew ^{[1
]}

机构：

[1] Stevens Inst Technol, Dept Chem Engn & Mat Sci, Hoboken, NJ 07030 USA

[2] Hackensack Univ, Med Ctr, Dept Pathol, Microbiol & Mol Diagnost Lab, Hackensack, NJ 07601 USA

[3] Seton Hall Univ, Hackensack Meridian Sch Med, Nutley, NJ 07110 USA

[4] Hackensack Univ, Med Ctr, Dept Pathol, Clin Virol Lab, Hackensack, NJ 07601 USA

来源：

ANALYST | 2020年 / 145卷 / 23期

关键词：

PLATFORM; LENSES; IMAGE;

D O I：

10.1039/d0an01231a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We use electron-beam patterned functional microgels to integrate self-reporting molecular beacons, dielectric microlenses, and solid-phase and/or solution-phase nucleic acid amplification in a viral-detection microarray model. The detection limits for different combinations of these elements range from 10(-10) M for direct target-beacon hybridization alone to 10(-18) M when all elements are integrated simultaneously.

引用

页码：7528 / 7533

页数：6

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