Analysis and comparison of serum proteomics of senescence accelerated mice

被引：0

作者：

Jiang Ning

Zhou Wen-Xia ^{[1
]}

Zhang Yong-Xiang

Zhang Xue-Min

Wang Jie

Liu Bing-Yu

机构：

[1] Chinese Acad Mil Med, Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China

[2] Natl Ctr Biomed Anal, Beijing 100850, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2006年 / 27卷 / 08期

关键词：

accelerated senescence mouse; serum proteomics; two dimensional gel electrophoresis;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

To analyze and compare differential serum proteins of senescence accelerated mice: SAM-prone/8 (SAMP 8) and SAM-resistance/1 (SAMR 1), the proteins of sera from 6 and 12 month-old SAMP 8 and age-matched SAMR 1 were separated, detected and analyzed. Compared with the same age SAMR 1, 15 proteins expressions in sera of six-month-old SAMP 8 were up-regulated, 3 proteins expressions down-regulated significantly and 7 proteins only expressed in SAMP 8 sera; 9 proteins expressions in sera of twelve-month-old SAMP 8 were up-regulated, 7 proteins expressions down-regulated significantly and 12 proteins only expressed in SAMP 8 sera. Using MALDI-TOF-MS, 19 proteins with significant changes were identified by peptide fingerprinting-map and the results searched in MASCOT database. The results showed that 6 proteins were only expressed in sera of six-month-old SAMP 8, 4 proteins only expressed in sera of twelve-month-old SAMP 8. In addition, there were 9 common proteins presenting both in sera of six and twelve-month-old SAMP 8. Our study showed that there were significant differences in serum protein expressions between SAMP 8 and SAMR I, and some differential proteins may result in pathological changes during the course of aging.

引用

页码：1462 / 1466

页数：5

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