COMPARATIVE STUDY ON ENZYMATIC HYDROLYSIS OF CELLULOSE

Identification and optimization of strains with high enzyme activity able to overcome constrains imposed by the cellulosic structure represents an important step in the development of new biotechnologies for bioethanol. this paper aims to reveal the advantages and disadvantages of the cellulase enzymes derived from two complet different microorganisms: Trichoderma ressei, a very known cellulase producers and Butyrivibrio fibrisolvens, a ruminal bacteria. Both organisms were inoculated under the same conditions (strict anaerobic, Sabouraud dextrose agar media, pH and temperature). The cellulose degradation was investigated by the time evolution of cellulase activity and the amount of reducing sugar released (glucose as standard) from carboxymethyl cellulose.