Two novel transposon delivery vectors based on mariner transposon for random mutagenesis of Bacillus thuringiensis

被引：4
|
作者：
Li, Mingshun
Li, Minglei
Yin, Wen
He, Jin
Yu, Ziniu [1 ]
机构：
[1] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2009年 / 78卷 / 02期
基金：
国家高技术研究发展计划(863计划);
关键词：
Bacillus thuringiensis; Mariner; Mutantagenesis; Transposon; IN-VITRO; HIMAR1-MARINER TRANSPOSON; TN10; TRANSPOSITION; BURSA-AUREALIS; ANTHRACIS; VIRULENCE; EXPRESSION; SUBTILIS; PROTEIN; GENOME;
D O I：
10.1016/j.mimet.2009.06.008
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Two new mariner transposon delivery vector systems, pMarA333 and pMarB333, had been constructed to randomly mutagenize Bacillus thuringiensis in vivo. The results showed that both systems could randomly insert into the genome of B. thuringiensis YBT881 (CCAM 020673). These systems could have potential for further construction of mutant libraries of B. thuringiensis and other Bacillus strains. (c) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：242 / 244
页数：3
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