Characterization of the upper pathway genes for fluorene metabolism in Terrabacter sp strain DBF63

被引:30
|
作者
Habe, H
Chung, JS
Kato, H
Ayabe, Y
Kasuga, K
Yoshida, T
Nojiri, H
Yamane, H
Omori, T
机构
[1] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan
[2] Akita Prefectural Univ, Fac Bioresource Sci, Dept Biotechnol, Akita, Japan
关键词
D O I
10.1128/JB.186.17.5938-5944.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Genes involved in the degradation of fluorene to phthalate were characterized in the fluorene degrader Terrabacter sp. strain DBF63. The initial attack on both fluorene and 9-fluorenone was catalyzed by DbfA to yield 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluorenone, respectively. The FlnB protein exhibited activities against both 9-fluorenol and 1,1a-dihydroxy-1-hydro-9-fluore none to produce 9-fluorenone and 2'-carboxy-2,3-dihydroxybiphenyl, respectively. FlnD is a heteromeric protein encoded byflnD1 and ORF16, being a member of the class III two-subunit extradiol dioxygenase. FlnE was identified as a serine hydrolase for the meta-cleavage products that yield phthalate.
引用
收藏
页码:5938 / 5944
页数:7
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