Most sprint interval training (SIT) research involves cycling as the mode of exercise and whether running SIT elicits a similar excess postexercise oxygen consumption (EPOC) response to cycling SIT is unknown. As running is a more whole-body-natured exercise, the potential EPOC response could be greater when using a running session compared with a cycling session. The purpose of the current study was to determine the acute effects of a running versus cycling SIT session on EPOC and whether potential sex differences exist. Sixteen healthy recreationally active individuals (8 males and 8 females) had their gas exchange measured over similar to 2.5 h under 3 experimental sessions: (i) a cycle SIT session, (ii) a run SIT session, and (iii) a control (CTRL; no exercise) session. Diet was controlled. During exercise, both SIT modes increased oxygen consumption (cycle: male, 1.967 +/- 0.343; female, 1.739 +/- 0.296 L.min(-1); run: male, 2.169 +/- 0.369; female, 1.791 +/- 0.481 L.min(-1)) versus CTRL (male, 0.425 +/- 0.065 L.min(-1); female, 0.357 +/- 0.067; P < 0.001), but not compared with each other (P = 0.234). In the first hour postexercise, oxygen consumption was still increased following both run (male, 0.590 +/- 0.065; female, 0.449 +/- 0.084) and cycle SIT (male, 0.556 +/- 0.069; female, 0.481 +/- 0.110 L.min(-1)) versus CTRL and oxygen consumption was maintained through the second hour postexercise (CTRL: male, 0.410 +/- 0.048; female, 0.332 +/- 0.062; cycle: male, 0.430 +/- 0.047; female, 0.395 +/- 0.087; run: male, 0.463 +/- 0.051; female, 0.374 +/- 0.087 L.min(-1)). The total EPOC was not significantly different between modes of exercise or males and females (P > 0.05). Our data demonstrate that the mode of exercise during SIT (cycling or running) is not important to O-2 consumption and that males and females respond similarly.