Dissecting the Genetic Basis of Resistance to Malaria Parasites in Anopheles gambiae

被引：83
|
作者：
Blandin, Stephanie A. [1 ,2 ]
Wang-Sattler, Rui [1 ,3 ]
Lamacchia, Marina [2 ]
Gagneur, Julien [1 ]
Lycett, Gareth [1 ]
Ning, Ye [1 ]
Levashina, Elena A. [2 ]
Steinmetz, Lars M. [1 ]
机构：
[1] European Mol Biol Lab, D-69117 Heidelberg, Germany
[2] INSERM, CNRS, UPR9022, U963, F-67084 Strasbourg, France
[3] Helmholtz Zentrum Munchen, Inst Epidemiol, D-85764 Munich, Germany
来源：
SCIENCE | 2009年 / 326卷 / 5949期
关键词：
COMPLEMENT-LIKE PROTEIN; QUANTITATIVE TRAIT LOCI; MELANIZATION RESPONSE; PLASMODIUM-BERGHEI; MOSQUITO; TEP1;
D O I：
10.1126/science.1175241
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The ability of Anopheles gambiae mosquitoes to transmit Plasmodium parasites is highly variable between individuals. However, the genetic basis of this variability has remained unknown. We combined genome-wide mapping and reciprocal allele-specific RNA interference (rasRNAi) to identify the genomic locus that confers resistance to malaria parasites and demonstrated that polymorphisms in a single gene encoding the antiparasitic thioester-containing protein 1 (TEP1) explain a substantial part of the variability in parasite killing. The link between TEP1 alleles and resistance to malaria may offer new tools for controlling malaria transmission. The successful application of rasRNAi in Anopheles suggests that it could also be applied to other organisms where RNAi is feasible to dissect complex phenotypes to the level of individual quantitative trait alleles.
引用
收藏
页码：147 / 150
页数：4
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