Concentration dependent effect of ethylene glycol on the structure and stability of holo α-lactalbumin: Characterization of intermediate state amidst soft interactions

被引：6

作者：

Raina, Neha ^{[1
,2
]}

Singh, Amit Kumar ^{[1
]}

Hassan, Md Imtaiyaz ^{[2
]}

Ahmad, Faizan ^{[2
]}

Islam, Asimul ^{[2
]}

机构：

[1] Shardo Univ, Dept Biotechnol, Greater Noida 201310, Uttar Pradesh, India

[2] Jamia Millia Islamia, Ctr Interdisciplinary Res Basic Sci, New Delhi 110025, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2020年 / 164卷

关键词：

Holo alpha-lactalbumin; Ethylene glycol; Protein stability; UV spectroscopy; Fluorescence spectroscopy; Circular dichroism; Protein folding; Molten globule; MOLTEN GLOBULE STATE; EXCLUDED-VOLUME; PROTEIN AGGREGATION; THERMAL STABILIZATION; GUANIDINIUM CHLORIDE; SECONDARY STRUCTURES; FUNCTIONAL-ACTIVITY; CROWDING AGENTS; CYTOCHROME-C; OLEIC-ACID;

D O I：

10.1016/j.ijbiomac.2020.07.224

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interior of the cell is crowded with different kinds of biological molecules with varying sizes, shapes and compositions which may affect physiological processes especially protein folding, protein conformation and protein stability. To understand the consequences of such a crowded environment, pH-induced unfolding of holo alpha-lactalbumin (holo alpha-LA) was studied in the presence of ethylene glycol (EG). The effect of EG on the folding and stability of holoa-LA in aqueous solution was investigated using several spectroscopic techniques. The results indicate that stabilization/destabilization of holo alpha-LA by EG is concentration- and pH-dependent. Low concentration of EG stabilizes the protein at pH near its pI. From the results of far-UV CD, UV-visible and ANS fluorescence, intermediate state (MG state) was characterized in the presence of high concentration of ethylene glycol. The results invoke a new mechanism for the formation of MG state identical to active component of BAMLET. MG state of holoa-LA has a direct implication to cancer therapy. MG state of alpha-LA in complex with specific type of lipid is a novel class of protein-based anti-cancer complexes that incorporate oleic acid and deliver it to the cancer cells. (C) 2020 Published by Elsevier B.V.

引用

页码：2151 / 2161

页数：11