BSA-boronic acid conjugate as lectin mimetics

被引：11

作者：

Narla, Satya Nandana ^{[1
]}

Pinnamaneni, Poornima ^{[1
]}

Nie, Huan ^{[2
]}

Li, Yu ^{[2
]}

Sun, Xue-Long ^{[1
]}

机构：

[1] Cleveland State Univ, Ctr Gene Regulat Hlth & Dis GRHD, Dept Chem Chem & Biomed Engn, Cleveland, OH 44115 USA

[2] Harbin Inst Technol, Sch Life Sci & Technol, Harbin 150006, Heilongjiang, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2014年 / 443卷 / 02期

基金：

美国国家科学基金会; 中国国家自然科学基金;

关键词：

Boronic acid; Bovine serum albumin; Carbohydrate; Lectin; Alizarin Red S; Surface plasmon resonance; MICROARRAY; GLYCOPEPTIDES; ENRICHMENT; POLYMERIZATION; NANOPARTICLES; GLYCOPROTEINS; RECOGNITION; STRATEGY; ARRAYS; CELLS;

D O I：

10.1016/j.bbrc.2013.12.006

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report bovine serum albumin (BSA)-boronic acid (BA) conjugates as lectin mimetics and their glyco-capturing capacity. The BSA-BA conjugates were synthesized by amidation of carboxylic acid groups in BSA with aminophenyl boronic acid in the presence of EDC, and were characterized by Alizarin Red S (ARS) assay and SDS-PAGE gel. The BSA-BA conjugates were immobilized onto maleimide-functionalized silica beads and their sugar capturing capacity and specificity were confirmed by ARS displacement assay. Further, surface plasmon resonance (SPR) analysis of the glyco-capturing activity of the BSA-BA conjugates was conducted by immobilizing BSA-BA onto SPR gold chip. Overall, we demonstrated a BSA-BA-based lectin mimetics for glyco-capturing applications. These lectin mimetics are expected to provide an important tool for glycomics and biosensor research and applications. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：562 / 567

页数：6

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