A new protocol for solubilization, refolding and purification of recombinant human granulocyte colony-stimulating factor in inclusion bodies

被引：0

作者：

Liu, Jia Hua ^{[1
]}

Wang, Chao Zhan ^{[1
]}

Geng, Xin Du ^{[1
]}

机构：

[1] NW Univ Xian, Key Lab Separat Sci Shaanxi Prov, Inst Modern Separat Sci, Xian 710069, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2006年 / 17卷 / 06期

关键词：

recombinant human granulocyte colony-stimulating factor; alkaline solution; solubilization of inclusion bodies; protein refolding;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Recombinant human granulocyte colony-stimulating factor (rhG-CSF) in inclusion bodies was solubilized by 8 mol/L urea solution and subsequently precipitated by acetone to improve its purity. After that, the precipitates were solubilized by sodium hydroxide solution containing 2 mol/L urea. Then the solubilized rhG-CSF was passed through a size exclusion chromatography for refolding and extensive purification, and further purified by a weak anion exchange chromatography. The purity and mass recovery of refolded rhG-CSF were 96.5% and 75.6%, respectively. The bioactivity was 8.4 x 10(7) IU/Mg.

引用

页码：799 / 802

页数：4

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