Realistic modeling of digital holographic microscopy

A self-contained platform for realistic modeling of digital holographic microscopy (DHM) is presented. Amplitude and phase samples, imaged in different architectures, can be modeled to produce numerical DHM holograms that include all the parameters that are present in real experiments, providing an accessible way for newcomers to have a first approach to this research field. The platform is based on considering the imaging arm of the DHM that produces the object wave as the result of the convolution process between the geometrical-optics image prediction of the sample with the point spread function introduced by diffraction. The DHM hologram is produced by the amplitude superposition of complex-valued object wave with a reference wave of arbitrary description. The sampling of the analytically produced DHM holograms is set from the input discretized image according to the specifications of the digital camera aimed to be used. The feasibility of the realistic platform is exemplified by contrasting the wrong results of a nonrealistic simulation with experimental results to show the need for using a complete realistic simulation like the one presented; further applications of the platform to numerical modeling speckle noise reduction over samples with controlled levels of roughness, and phase-shifting DHM techniques, are included. (C) 2020 Society of Photo-Optical Instrumentation Engineers (SPIE)