Epimeric separation of L-ascorbic acid and D-isoascorbic acid by capillary zone electrophoresis

被引:19
|
作者
Liao, TC
Wu, JSB
Wu, MC
Chang, HM [1 ]
机构
[1] Natl Taiwan Univ, Grad Inst Food Sci & Technol, Taipei 106, Taiwan
[2] Pingtung Univ Sci & Technol, Dept Food Sci & Technol, Pingtung, Taiwan
关键词
epimeric separation; L-ascorbic acid (L-AA); D-isoascorbic acid (D-IAA); capillary zone electrophoresis (CZE);
D O I
10.1021/jf990399e
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Capillary zone electrophoresis (CZE) was used for separation of L-ascorbic acid (L-AA) and D-isoascorbic acid (D-IAA) in a model system. The effects of borate buffer concentration (0.05-0.25 M) and pH (pH 7.5-9.0) on migration time, resolution (Rs), and theoretical plates (N) were investigated. The migration times of L-AA and D-IAA increased with the increasing pH of carrier electrolyte (0.2 borate buffer), and the resolutions (Rs) of L-AA and D-IAA were calculated to be 12.98 at pH 9.0. Concentrations of borate buffer (pH 9.0) increased the Rs values of L-AA and D-IAA, and buffer concentrations >0.1 M were found to be effective for separation of L-AA and D-IAA. Methanol in the carrier electrolyte was also influential in improving the separation of L-AA and D-IAA, which increased with the increasing concentrations (0-10%) of methanol. The optimal separation conditions for L-AA and D-IAA were as follows: carrier electrolyte, 0.2 M borate buffer (pH 9.0); applied voltage, 25 kV, with an uncoated fused silica capillary, 75 mu m (i.d.) x 57 cm.
引用
收藏
页码:37 / 41
页数:5
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