Lysis of Mammalian and Sf9 Cells

被引：1
|
作者：
Kavran, Jennifer M. [1 ]
Leahy, Daniel J. [1 ]
机构：
[1] Johns Hopkins Univ, Sch Med, Dept Biophys & Biophys Chem, Baltimore, MD 21205 USA
来源：
LABORATORY METHODS IN ENZYMOLOGY: PROTEIN PT A | 2014年 / 536卷
关键词：
D O I：
10.1016/B978-0-12-420070-8.00004-0
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Use a French press to disrupt mammalian or Sf9 cells and generate a clarified lysate for subsequent use in protein purification.
引用
收藏
页码：47 / 52
页数：6
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