Rat embryo culture using rabbit serum as a medium for developmental toxicity studies

被引：0

作者：

Nakajima, M ^{[1
]}

Sasaki, M ^{[1
]}

Kobayashi, Y ^{[1
]}

Ohno, Y ^{[1
]}

Usami, M ^{[1
]}

机构：

[1] NATL INST HLTH SCI,DIV PHARMACOL,TOKYO 158,JAPAN

来源：

JOURNAL OF APPLIED TOXICOLOGY | 1997年 / 17卷 / 03期

关键词：

postimplantation rat embryo culture; rat serum; rabbit serum; developmental toxicity;

D O I：

10.1002/(SICI)1099-1263(199705)17:3<185::AID-JAT428>3.0.CO;2-#

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

The usefulness of rabbit serum as a culture medium for postimplantation rat embryos was examined, Rat embryos at day 9.5 of gestation were cultured in a mixture of rat and rabbit sera at various ratios (v/v) for 48 h. In 100% rat serum, a usual medium, rat embryos grew well. On the contrary, rat embryos died with little growth in 100% rabbit serum. In 75% rabbit and 25% rat sera, rat embryos grew but were morphologically abnormal. In 50% rabbit and 50% rat serum, however, rat embryos grew well showing no morphological abnormalities, as in 100% rat serum. It was concluded from these results that rabbit serum could be used at a proportion up to 50% as a medium for postimplantation rat embryo culture in a mixed form with rat serum. The rat embryo culture using rabbit serum as a medium would he useful in developmental toxicity studies, especially those involving species differences and toxicokinetics. (C) 1997 by John Whey & Sons, Ltd.

引用

页码：185 / 188

页数：4

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