Detection of Haemophilus influenzae type b by real-time PCR

被引:24
|
作者
Marty, A
Greiner, O
Day, PJR
Gunziger, S
Mühlemann, K
Nadal, D
机构
[1] Univ Zurich, Childrens Hosp, Div Infect Dis, CH-8032 Zurich, Switzerland
[2] Univ Zurich, Childrens Hosp, Div Oncol, CH-8032 Zurich, Switzerland
[3] Univ Bern, Inst Med Microbiol, CH-3010 Bern, Switzerland
关键词
D O I
10.1128/JCM.42.8.3813-3815.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A real-time PCR assay targeting the capsulation locus of Haemophilus influenzae type b (Hib) was developed. The linear detection range was from 1 to 10(6) microorganisms per reaction mixture. No H. influenzae other than Hib or any other control bacteria typically found in the upper respiratory tract was detected.
引用
收藏
页码:3813 / 3815
页数:3
相关论文
共 50 条
  • [1] Real-Time PCR Detection of Haemophilus influenzae: Non-Typeable Strains
    Law, D. K. S.
    Zhou, J.
    Shuel, M. L.
    Tsang, R. S. W.
    [J]. 26TH INTERNATIONAL CONGRESS OF CHEMOTHERAPY AND INFECTION (ICC), 2009, : 139 - 142
  • [2] Real-Time PCR Detection of Haemophilus influenzae: Non-Typeable Strains
    Law, D. K. S.
    Zhou, J.
    Shuel, M. L.
    Tsang, R. S. W.
    [J]. NEW APPROACHES IN CORONARY ARTERY DISEASE: PROCEEDINGS OF THE 8TH INTERNATIONAL CONGRESS ON CORONARY ARTERY DISEASE, 2009, : 139 - 142
  • [3] Simultaneous identification of Haemophilus influenzae and Haemophilus haemolyticus using real-time PCR
    Price, Erin P.
    Harris, Tegan M.
    Spargo, Jessie C.
    Nosworthy, Elizabeth
    Beissbarth, Jemima
    Chang, Anne B.
    Smith-Vaughan, Heidi C.
    Sarovich, Derek S.
    [J]. FUTURE MICROBIOLOGY, 2017, 12 (07) : 585 - 593
  • [4] Identifying Haemophilus haemolyticus and Haemophilus influenzae by SYBR Green real-time PCR
    Latham, Roger
    Zhang, Bowen
    Tristram, Stephen
    [J]. JOURNAL OF MICROBIOLOGICAL METHODS, 2015, 112 : 67 - 69
  • [5] Real-time PCR for determining capsular serotypes of Haemophilus influenzae
    Maaroufi, Younes
    De Bruyne, Jean-Marc
    Heymans, Corine
    Crokaert, Francoise
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (07) : 2305 - 2308
  • [6] Direct Real-Time PCR for the Detection and Serotyping of Haemophilus influenzae without DNA Extraction
    Marasini, Daya
    Whaley, Melissa J.
    Jenkins, Laurel T.
    Hu, Fang
    Jiang, Wenxin
    Topaz, Nadav
    Chen, Alex
    Schmink, Susanna
    Dolan Thomas, Jennifer
    Harcourt, Brian H.
    Marjuki, Henju
    Wang, Xin
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2022, 60 (04)
  • [7] Real-time PCR detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae DNA in clinical specimens
    Vackova, Z.
    Lzicarova, D.
    Stock, N. K.
    Kozakova, J.
    [J]. EPIDEMIOLOGIE MIKROBIOLOGIE IMUNOLOGIE, 2015, 64 (04): : 222 - 230
  • [8] Detection of bacterial pathogens in Mongolia meningitis surveillance with a new real-time PCR assay to detect Haemophilus influenzae
    Wang, Xin
    Mair, Raydel
    Hatcher, Cynthia
    Theodore, M. Jordan
    Edmond, Karen
    Wu, Henry M.
    Harcourt, Brian H.
    Carvalho, Maria da Gloria S.
    Pimenta, Fabiana
    Nymadawa, Pagbajab
    Altantsetseg, Dorjpurev
    Kirsch, Mariah
    Satola, Sarah W.
    Cohn, Amanda
    Messonnier, Nancy E.
    Mayer, Leonard W.
    [J]. INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2011, 301 (04) : 303 - 309
  • [9] Detection of Haemophilus influenzae type B DNA in a murine pneumonia model by in situ PCR
    Hu, Huili
    He, Lejian
    Hu, Yiyun
    Jiang, Min
    Yao, Kaihu
    Yang, Yonghong
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 57 (10) : 1282 - 1287
  • [10] Quantitative detection of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis in lower respiratory tract samples by real-time PCR
    Kais, Madeleine
    Spindler, Carl
    Kalin, Mats
    Ortqvist, Ake
    Giske, Christian G.
    [J]. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2006, 55 (03) : 169 - 178