Determination of Sudan Residues in Sausage by Matrix Solid-Phase Dispersion and High-Performance Liquid Chromatography

A method based on matrix solid-phase dispersion and high-performance liquid chromatography was applied to the determination of four Sudan red residues in sausage. The proposed method required only 0.5 g sample. The neutral alumina was used as the dispersant sorbent while n-hexane containing 10% (v/v) acetone was used as the eluting solvent. The recoveries in samples ranged from 76.4 to 111.0% and relative standard deviations were <10.2% with different spiked levels of 0.05-1.50 mg kg(-1). Detection and quantification limits were 5-19 and 18-39 mu g kg(-1), respectively, with linear calibration curves extending up to 2.50 mg g(-1). The results demonstrate that the method can be successfully applied with acceptable recoveries to a broad range of target analytes within a diverse range of sausage.