Histone deacetylase inhibitor trichostatin A modulates cell cycles in A2780 human ovarian cancer cell lines

被引：0

作者：

Ma, X. X. ^{[1
]}

Jin, Y. N. ^{[1
]}

Miao, Q. ^{[1
]}

Li, S. ^{[1
]}

He, Y. Q. ^{[1
]}

Lv, H. W. ^{[1
]}

机构：

[1] China Med Univ, Shengjing Hosp, Dept Obstet & Gynecol, Shenyang 110004, Peoples R China

来源：

EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY | 2009年 / 30卷 / 03期

关键词：

TSA; A2780; cells; Flow cytometry; cell cycle; P21(WAF/CIPI); TRANSCRIPTION; SP1;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Purpose of investigation: To analyze the effect and mechanism of TSA on cell cycles in human ovarian cancer cells. Methods: cells were cultured in RPMI 1640 supplemented. Flow cytometry analysis and RT-PCR were used to examine the distribution of cell cycles and the level of P21(WAF/CIPI) mRNA. Results: TSA induced increase of the G(2)/M phase accompanied by decrease of the S phase and enhanced level of P21(WAF/CIPI) mRNA in a concentration and time-dependent manner in A2780 cells. Conclusion: Trichostatin A affects the activity of cyclin-dependent kinase through increased expression of P21(WAF/CIPI) mRNA. TSA causes A2780 cell blockage in the G(2)/M phase and inhibits cell proliferation of A2780 cells. The minimum level of active TSA is 100nM and the minimum time is 12 hours. The effect relies on time and concentration.

引用

页码：263 / 266

页数：4

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