Limnological variables from 30 lakes situated along the proposed Mackenzie Gas Project pipeline route in the Canadian Northwest Territories were examined. Sampled lakes were shallow (Z(max) = 1.5-30 m; mean depth = 6.6 m), generally nutrient poor (TP qfien <= 10 mu g L-1), and alkaline (mean pH = 8.2). Floodplain lakes located within Arclic-tundra watersheds tended to have higher conductivity and major tons (Na, Cl, Ca, SO4) concentrations relative to non-flooded Arctic-tundra or forest-tundra lakes, reflecting differences in vegetation, elevation and most importantly, proximity to the Mackenzie River. The first two axes of a principal component analysis explained 56.1% of the variance in the environmental data. Variables most strong v associated with the first principal component avis were latitude, elevation, dissolved oxygen. temperature, conductivity and turbidity while the second principal component axis represented gradients of nutrients and Chlorophyll-a (Chl-a). Factors affecting Chl-a varied among the different ecological Zones. This study provides reference data for future monitoring of potential effects of development and warming in the Arctic.