Characterization of Potato spindle tuber viroid (PSTVd) incidence and new variants from ornamentals

被引:8
|
作者
Matousek, Jaroslav [1 ]
Piernikarczyk, Rajen J. J. [4 ]
Dedic, Petr [2 ]
Mertelik, Josef [3 ]
Uhlirova, Katerina [1 ]
Duraisamy, Ganesh S. [1 ]
Orctova, Lidmila [1 ]
Kloudova, Katerina [3 ]
Ptacek, Jiri [2 ]
Steger, Gerhard [4 ]
机构
[1] BC ASCR, Vvi, Inst Plant Mol Biol, Ceske Budejovice 37005, Czech Republic
[2] Potato Res Inst, Havlickuv Brod, Czech Republic
[3] Silva Tarouca Res Inst Landscape & Ornamental Gar, Pruhonice, Czech Republic
[4] Univ Dusseldorf, Inst Phys Biol, D-40204 Dusseldorf, Germany
关键词
Viroid pathogenicity; Real-time PCR; Biolistic inoculation of plants; Solanum jasminoides; Solanum tuberosum; Solanum lycopersicum; SOLANUM-JASMINOIDES; MOLECULAR ANALYSIS; PLANTS; LOCALIZATION; TRANSMISSION; NUCLEI; VIRUS; RNAS;
D O I
10.1007/s10658-013-0304-6
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
We analyzed the spreading and persistence of PSTVd variants in several ornamentals in the territory of the Czech Republic. The pool of PSTVd variants detected in Solanum jasminoides, S. muricatum, Datura sp. and Brugmansia sp. was biolistically transferred to Matricaria chamomilla, Argyranthemum frutescens and Diascia sp., species which we found as sensitive hosts for PSTVd from ornamentals. The PSTVd pool showed sequence changes and increased variation after its transfer to potato, suggesting a wide adaptation potential of PSTVd in this crop. Potato exhibited genotype-dependent leaf and spindle tuber symptoms, when inoculated with the sap from S. jasminoides infected with the predominant and sequence-stable PSTVd-S1.
引用
收藏
页码:93 / 101
页数:9
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