Inheritance of C3-C4 intermediate photosynthesis in reciprocal hybrids between Moricandia arvensis (C3-C4) and Brassica oleracea(C3) that differ in their genome constitution

To elucidate the genetic mechanisms underlying C-3-C-4 intermediate photosynthesis, we investigated the structural and photosynthetic characteristics of leaves of reciprocal hybrids between the C-3-C-4 intermediate species Moricandia arvensis (L.) DC. (MaMa) and the C-3 species Brassica oleracea L. (cabbage; CC), which differ in genome constitution. Moricandia arvensis bundle sheath (BS) cells included many centripetally located chloroplasts and mitochondria, whereas those of cabbage had few organelles. Hybrid leaves were structurally intermediate between those of the parents and showed stronger intermediate C-3-C-4 features as the proportion of the Ma genome increased. The P-protein of glycine decarboxylase (GDC) was confined mainly to BS mitochondria, in M. arvensis, but accumulated more in the mesophyll (M) of cabbage. In the hybrids, the accumulation of GDC in BS cells increased with an increasing Ma:C ratio. Hybrids exhibited gradients in structural and biochemical features, even in reciprocal crosses. The CO2 compensation point of reciprocal hybrids with high Ma:C ratios was lower than that of cabbage but higher than that of M. arvensis. Thus, the structural and biochemical features in hybrid leaves reduced photorespiration. Moricandia arvensis had a higher photosynthetic rate than cabbage, but the photosynthetic rates of hybrids were intermediate between those of the parents or comparable to that of M. arvensis. Our results demonstrate that the C-3-C-4 intermediate characteristics are inherited based on the ratio of the parent genomes, and that there is no evidence of cytoplasmic inheritance in these characteristics.