Cocultivation of hematopoietic cells in a fluidized bed reactor

被引：0
|
作者：
Schroder, B
Herfurth, C
Biselli, M
Schmoll, HJ
Link, H
Ebell, W
Wandrey, C
机构：
来源：
ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 8 | 1997年
关键词：
bone marrow; cocultivation; fluidized bed reactor; hematopoietic cells; stromal cells; immobilization; porous microcarriers;
D O I：
暂无
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
To expand bone marrow and to imitate its natural environment we have developed a three dimensional cultivation in microcarriers in a fluidized bed reactor. To immobilize the bone marrow cells, a feeder layer of stromal cells is established. After inoculation of the bone marrow preferentially the progenitor cells attached to the feeder layer and the cell density increased twelvefold.
引用
收藏
页码：137 / 141
页数：5
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