One RNA aptamer sequence, two structures: a collaborating pair that inhibits AMPA receptors

被引：20

作者：

Huang, Zhen ^{[1
,2
]}

Pei, Weimin ^{[1
,2
]}

Han, Yan ^{[1
,2
]}

Jayaseelan, Sabarinath ^{[1
,2
]}

Shekhtman, Alexander ^{[1
,2
]}

Shi, Hua ^{[3
]}

Niu, Li ^{[1
,2
]}

机构：

[1] SUNY Albany, Dept Chem, Albany, NY 12222 USA

[2] SUNY Albany, Neurosci Res Ctr, Albany, NY 12222 USA

[3] SUNY Albany, Dept Biol Sci, Albany, NY 12222 USA

来源：

NUCLEIC ACIDS RESEARCH | 2009年 / 37卷 / 12期

基金：

美国国家卫生研究院;

关键词：

CHANNEL-OPENING KINETICS; RIBOZYME; SECONDARY; STABILITY;

D O I：

10.1093/nar/gkp284

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA is ideally suited for in vitro evolution experiments, because a single RNA molecule possesses both genotypic (replicable sequence) and phenotypic (selectable shape) properties. Using systematic evolution of ligands by exponential enrichment (SELEX), we found a single 58-nt aptamer sequence that assumes two structures with different functions, both of which are required to inhibit the GluR2 AMPA receptor channel. Yet, the two structures, once formed during transcription, appear to be incapable of interconverting through unfolding and refolding, presumably due to their extraordinary structural stability. Thus, our results suggest more broadly that natural RNA molecules can evolve to acquire alternative structures and associated functions. Such divergence of RNA phenotype may precede gene duplication at the genome level.

引用

页码：4022 / 4032

页数：11

共 19 条

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Huang, Zhen
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[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2024, 63 (37)
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