Children today face significant challenges in response to living in a globalised world and the predicted environmental threats to the planet. However, implementing environmental and sustainability education (ESE) within schools can be complex and is often ignored within initial teacher education (ITE). In response, this project aimed to evaluate pedagogies for developing transformative learning for ESE within the context of university-based ITE. We ran a conference for trainee teachers informed by critical, participatory and arts-based pedagogic approaches. Alongside this, we undertook an interpretive case study, collecting data through questionnaires, focus groups and in-conference spoken reflection. Findings suggest trainee teachers gained a more nuanced understanding of the value and nature of ESE, as well as more affective and informed engagement with it, across the conference. Implications for ESE within ITE focus around the potential of pedagogies which provide affective experiences, in particular participatory and arts-based pedagogical approaches, for inspiring and empowering trainee teachers to enter into schools and develop their own ESE practice as classroom teachers.