Isolation of ob gene and its expression in E-coli

被引：0

作者：

Zou, MJ ^{[1
]}

Wang, JX ^{[1
]}

Wang, LH ^{[1
]}

Duan, JB ^{[1
]}

Cai, X ^{[1
]}

机构：

[1] Chinese Acad Med Sci, Inst Basic Med Sci, Beijing 100850, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 2000年 / 27卷 / 01期

关键词：

ob gene; PCR; clone; expression;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The exon 2, 3 of ob gene were isolated from chromosome of human peripheral leucocytes by means of PCR technology. The full-length coding region of ob gene was;obtained by in vitro splicing with ligase. DNA sequencing confirmed that the isolated ob coding sequence is identical to that reported in the literature. The coding sequence for mature OB protein was amplified by PCR, and then cloned into an expression vector pBV220. As a result, an expression bacteria strain was obtained. The expressed product was preliminarily purified for further study.

引用

页码：91 / 93

页数：3

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