Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in hybrid organic-inorganic film of chitosan/sol-gel/carbon nanotubes

被引：78
|
作者：
Kang, Xinhuang [1 ,2 ]
Wang, Jun [1 ]
Tang, Zhiwen [1 ]
Wu, Hong [1 ]
Lin, Yuehe [1 ]
机构：
[1] Pacific NW Natl Lab, Richland, WA 99352 USA
[2] Guangdong Ocean Univ, Coll Sci, Zhanjiang 524088, Peoples R China
来源：
TALANTA | 2009年 / 78卷 / 01期
关键词：
Direct electrochemistry; Horseradish peroxidase; Multi-walled carbon nanotubes; Sol-gel; DIRECT ELECTRON-TRANSFER; GLASSY-CARBON ELECTRODE; GOLD NANOPARTICLE NANOCOMPOSITE; GLUCOSE-OXIDASE; COMPOSITE FILM; CYTOCHROME-C; HEME-PROTEINS; BIOSENSOR; ENZYMES; HYDROGEL;
D O I：
10.1016/j.talanta.2008.10.063
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A hybrid organic-inorganic nanocomposite film of chitosan/sol-gel/multi-walled carbon nanotubes was constructed for the immobilization of horseradish peroxidase (HRP). This film was characterized by scanning electron microscopy. Direct electron transfer (DET) and bioelectrocatalysis of HRP incorporated into the composite film were investigated. The results indicate that the film can provide a favorable microenvironment for HRP to perform DET on the surface of glassy carbon electrodes with a pair of quasi-reversible redox waves and to retain its bioelectrocatalytic activity toward H2O2. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码：120 / 125
页数：6
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