The reaction of copper in cytochrome oxidase with cytochrome c in rat brain in situ

被引:0
|
作者
Nomura, Y [1 ]
Fujii, F [1 ]
Matsunaga, A [1 ]
Tamura, M [1 ]
机构
[1] Hokkaido Univ, Res Inst Elect Sci, Div Biophys, Sapporo, Hokkaido 060, Japan
关键词
copper; heme a + a(3); cytochrome oxidase; cytochrome c; rat brain in situ; electron transfer;
D O I
10.3109/00207459808986445
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The role of copper of cytochrome oxidase in the electron transfer sequence proposed in vitro was investigated by spectroscopically monitoring the steady-state redox changes of cytochrome c, copper and heme a + a(3) of cytochrome oxidase in the perfused rat head. The percent oxidation of copper and cytochrome c in the aerobic-anaerobic transition did not show a straight-line relationship, As compared with copper, cytochrome c was most reduced, followed by heme a + a(3). These results suggested that copper first accepted an electron from heme a rather than cytochrome c in the mitochondrial membrane, unlike the electron transfer sequence proposed for purified enzymes. The orientation between cytochrome c and cytochrome oxidase would appear to be controlled primarily by the membrane structure.
引用
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页码:205 / 212
页数:8
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