Rapid differentiation of Old World Leishmania species by LightCycler polymerase chain reaction and melting curve analysis

被引：91
|
作者：
Nicolas, L [1 ]
Milon, G [1 ]
Prina, E [1 ]
机构：
[1] Inst Pasteur, Unite Immunophysiol & Parasitisme Intracellulaire, F-75724 Paris 15, France
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2002年 / 51卷 / 03期
关键词：
LightCycler; species-specific PCR; Leishmania;
D O I：
10.1016/S0167-7012(02)00099-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A LightCycler real-time polymerase chain reaction (PCR) assay has been developed to detect and differentiate four of the main Leishmania species of the Old World. The assay is based on fluorescence melting curve analysis of PCR products generated from the minicircles of kinetoplast DNA. According to the melting temperature, which is a function of GC/AT ratio, length and nucleotide sequences of the amplified product, Leishmania major was differentiated from L. donovani and from L. tropica and L. infantum. Melting curves analysis offers a rapid alternative for identification of species in diagnostic or epidemiological Studies of leishmaniasis or asymptomatic parasitism. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：295 / 299
页数：5
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