GuideScan software for improved single and paired CRISPR guide RNA design

被引：151

作者：

Perez, Alexendar R. ^{[1
,2
,3
]}

Pritykin, Yuri ^{[1
]}

Vidigal, Joana A. ^{[2
]}

Chhangawala, Sagar ^{[1
,3
]}

Zamparo, Lee ^{[1
]}

Leslie, Christina S. ^{[1
]}

Ventura, Andrea ^{[2
]}

机构：

[1] Mem Sloan Kettering Canc Ctr, Computat Biol Program, 1275 York Ave, New York, NY 10021 USA

[2] Mem Sloan Kettering Canc Ctr, Canc Biol & Genet Program, 1275 York Ave, New York, NY 10021 USA

[3] Cornell Univ, Weill Cornell Grad Sch Med Sci, New York, NY 10021 USA

来源：

NATURE BIOTECHNOLOGY | 2017年 / 35卷 / 04期

关键词：

GENETIC SCREENS; HUMAN-CELLS; IN-VIVO; GENOME; SPECIFICITY; NUCLEASES; SYSTEM; DNA;

D O I：

10.1038/nbt.3804

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs that are more specific than those designed by existing tools.

引用

页码：347 / +

页数：5

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