Toxin-binding properties of cytochrome P450 in Saccharomyces cerevisiae and Kluyveromyces marxianus

被引：5
|
作者：
Engler, KH
Kelly, SL
Coker, RD
Evans, IH
机构：
[1] Univ Greenwich, London SE18 6PF, England
[2] Univ Wales, Inst Biol Sci, Aberystwyth SY23 3DA, Dyfed, Wales
[3] Nat Resources Inst, Chatham ME4 4TB, Kent, England
来源：
BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 01期
关键词：
bioassay; cytochrome P450; Kluyveromyces marxianus; mycotoxins; Saccharomyces cerevisiae;
D O I：
10.1023/A:1005650217261
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Microsomes from Kluyveromyces marxianus GK1005 examined by carbon monoxide difference spectroscopy showed no evidence of cytochrome P450, in contrast to microsomes isolated from a control strain of Saccharomyces cerevisiae. Benzo[a]pyrene produced a typical Type I-binding spectrum with microsomes of both yeasts, with K-s values of 82 mu M (S. cerevisiae) and 70 mu M (K. marxianus). While aflatoxin B-1 generated a typical Type I-binding spectrum with microsomes from S. cerevisiae (K-s of 178 mu M), the toxin did not produce a recognisable binding spectrum with microsomes from K. marxianus.
引用
收藏
页码：3 / 8
页数：6
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