On-line rapid purification of [13N]N2 gas for visualization of nitrogen fixation and translocation in nodulated soybean

被引：6

作者：

Yin, Yong-Gen ^{[1
]}

Ishii, Satomi ^{[1
]}

Suzui, Nobuo ^{[1
]}

Igura, Masato ^{[1
,3
]}

Kurita, Keisuke ^{[1
,4
]}

Miyoshi, Yuta ^{[1
,2
]}

Nagasawa, Naotsugu ^{[1
]}

Taguchi, Mitsumasa ^{[1
]}

Kawachi, Naoki ^{[1
]}

机构：

[1] Natl Inst Quantum & Radiol Sci & Technol QST, Takasaki Adv Radiat Res Inst, Takasaki, Gunma 3701292, Japan

[2] Japan Soc Promot Sci, Tokyo, Japan

[3] Natl Agr & Food Res Org NARO, Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan

[4] Japan Atom Energy Agcy, Mat Sci Res Ctr, Tokai, Ibaraki 3191195, Japan

来源：

APPLIED RADIATION AND ISOTOPES | 2019年 / 151卷

关键词：

N-13]N-2; Nitrogen fixation; Nodule; PETIS; Purification; ROOT-NODULES; N-13; ASSIMILATION; N-15(2); VENTILATION; TRANSPORT; SYSTEM;

D O I：

10.1016/j.apradiso.2019.05.034

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Accurate analysis of N fixation in leguminous crops requires determination of N utilization within an intact plant; however, most approaches require tissue disassembly. We developed a simple and rapid technique to generate high-purity and high-yield [N-13]N-2 gas and obtained real-time images of N fixation in an intact soybean plant. The purification efficiency was similar to 81.6% after decay correction. Our method provides accurate signals of N Fixation and allows free changes to the tracer gas composition to suit different experimental designs.

引用

页码：7 / 12

页数：6

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