Differential Action of trans-10, cis-12 Conjugated Linoleic Acid on Adipocyte Differentiation of Ovine and 3T3-L1 Preadipocytes

被引：1
|
作者：
Iga, T. [1 ]
Satoh, T. [1 ]
Yamamoto, S. [1 ]
Fukui, K. [1 ]
Song, S. H. [2 ]
Choi, K. C. [3 ]
Roh, S. G. [2 ]
Sasaki, S. [1 ]
机构：
[1] Shinshu Univ, Fac Agr, Dept Food Prod Sci, Nagano 3994598, Japan
[2] Tohoku Univ, Dept Anim Physiol, Grad Sch Agr Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan
[3] Natl Inst Anim Sci, Cheonan 330801, South Korea
来源：
ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | 2009年 / 22卷 / 11期
关键词：
trans-10; cis-12; CLA; Adipocyte; Sheep; PROLIFERATOR-ACTIVATED RECEPTORS; ADIPOSE-TISSUE; CLA ISOMERS; METABOLISM; ADIPOGENESIS; PROTEINS; MUSCLE; RAT;
D O I：
10.5713/ajas.2009.80475
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
Trans-10, cis-12 conjugated linoleic acid (CLA) has been reported to inhibit the adipocyte differentiation of preadipocytes in non-ruminant animals (mice, rat, and human). However, the effects of trans-10, cis-12 CLA have not been clear in ruminants. The objective of this study was to investigate the effects of trans-10, cis-12 CLA on adipocyte differentiation of ovine preadipocytes. Differentiation of these preadipocytes was facilitated by treatment with trans-10, cis-12 CLA. Trans-10, cis-12 CLA increased the number and size of oil red O-stainable lipid drops as well as the levels of GPDH activity. PPAR-gamma 2 and adipophilin mRNA, adipogenic marker genes, were increased by treatment with trans-10, cis-12 CLA. This result was different from that observed with 3T3-L1 preadipocytes, a clonal cell line derived from rodents. Furthermore. trans-10, cis-12 CLA alone induced the adipocyte differentiation of ovine preadipocytes in differentiation-induction medium without troglitazone. These results suggest that CLA is an inducer and regulator in adipocyte differentiation of ovine preadipocytes, with species differences between ovine and rodent preadipocytes.
引用
收藏
页码：1566 / 1573
页数：8
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