Biological and molecular determination for resistance to Tomato spotted wilt virus (TSWV) in F2 population of long-type pepper (Capsicum annuum L.)

Pepper is one of the major vegetable crops grown in world. Viral diseases, causing significant yield losses, are one of the limiting factors of successful production. Tomato spotted wilt virus (TSWV) is one of the most common virus diseases in pepper. In sweet pepper, the Tsw gene, originally described in Capsicum chinense, has been widely used as an efficient gene for TSWV resistance. Resistance gene is determined by a single dominant gene. CAPS marker is used in molecular breeding program. The CAPS marker is tightly linked to Tsw and is helpful for marker assisted selection. In this study, it is aimed to determine resistance gene transferred to long pepper by using TSWV resistant pepper lines and cultivars. In breeding program, mechanical inoculation method to biologically identify and CAPS marker for molecular analysis are used for determination of resistance. In breeding study, three resistant lines and one susceptible line as parents are used. F-2 homozygous resistant individuals identified by using molecular marker are transferred into greenhouse.