Optical monitoring of therapeutic drugs with a novel fluorescence-based POCT device

被引：16
|
作者：
Berrettoni, C. [1 ,3 ]
Berneschi, S. [1 ]
Bernini, R. [2 ]
Giannetti, A. [1 ]
Grimaldi, I. A. [2 ]
Persichetti, G. [2 ]
Testa, G. [2 ]
Tombelli, S. [1 ]
Trono, C. [1 ]
Baldini, F. [1 ]
机构：
[1] CNR, Inst Appl Phys Nello Carrara, Via Madonna del Piano 10, I-50019 Sesto Fiorentino, FI, Italy
[2] CNR, Inst Electromagnet Sensing Environm, I-80124 Naples, Italy
[3] Univ Siena, Dept Informat Engn & Math, I-53100 Siena, Italy
来源：
28TH EUROPEAN CONFERENCE ON SOLID-STATE TRANSDUCERS (EUROSENSORS 2014) | 2014年 / 87卷
关键词：
immunosuppressants; tacrolimus; optical biochip; fluorescence anisotropy; TACROLIMUS; BLOOD;
D O I：
10.1016/j.proeng.2014.11.732
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
A novel optical biochip for immunosuppressants detection in transplanted patients is described. The optical working of the chip, based on total internal reflection fluorescence and consisting of two bonded polymeric parts, a Zeonex 330 slide and a Topas foil was demonstrated. In parallel, a heterogeneous competitive immunoassay for tacrolimus was implemented using a thirteen-microchannel optical chip interrogated by an in-house optical platform based on fluorescence anisotropy and the bioassay conditions were optimized: a limit of detection of 0.11 ng/mL, a limit of quantification of 0.57 ng/mL and a coefficient of variation of 25% were achieved. (C) 2014 The Authors. Published by Elsevier Ltd.
引用
收藏
页码：392 / 395
页数：4
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