Functional Characterization of the alb1 Orthologue Gene in the Ochratoxigenic Fungus Aspergillus carbonarius (AC49 strain)

被引：8

作者：

Gerin, Donato ^{[1
]}

Gonzalez-Candelas, Luis ^{[2
]}

Ballester, Ana-Rosa ^{[2
]}

Pollastro, Stefania ^{[1
,3
]}

Angelini, Rita Milvia De Miccolis ^{[1
,3
]}

Faretra, Francesco ^{[1
,3
]}

机构：

[1] Univ Bari Aldo Moro, Dept Soil Plant & Food Sci, Via Amendola 165-A, I-70126 Bari, Italy

[2] CSIC, IATA, Calle Agustin Escardino 7, Valencia 46980, Spain

[3] SELGE Network Publ Res Labs, Via Amendola 165-A, I-70126 Bari, Italy

来源：

TOXINS | 2018年 / 10卷 / 03期

关键词：

melanin; alb1; OTA partitioning; gene disruption; conidiation; sclerotia; SECONDARY METABOLISM; MELANIN BIOSYNTHESIS; NIDULANS; CONTAMINATION; SPORULATION; FLAVUS;

D O I：

10.3390/toxins10030120

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Aspergillus carbonarius, belonging to the group Nigri, is the main species responsible for contamination by ochratoxin A (OTA) in grapes and derivative products. OTA can accumulate in the mycelium and in black conidia of the fungus and released into the matrix. Here, we have deleted in A. carbonarius the alb1 orthologue gene of A. fumigatus, involved in melanin biosynthesis. Three A. carbonarius D alb1 mutants were characterized for morphologic traits and OTA production on different media and temperatures. D alb1 mutants showed a fawn color of conidia associated with a significant reduction of the conidiogenesis and a statistically significant increase (p <= 0.01) of total OTA production as compared to the wild type (WT) strain. The alb1 gene somehow affected OTA partitioning since in D alb1 mutants OTA amount was lower in conidia and was more abundantly secreted into the medium as compared to the WT. On grape berries the D alb1 mutants and the WT caused lesions with similar sizes but OTA amount in berry tissues was higher for the mutants. These results demonstrate that A. carbonarius conidia pigmentation is largely dependent on polyketide biosynthesis. The gene is not directly involved in virulence and its deletion affects morphological features and OTA production in the fungus.

引用

页数：16