Prevalence of Cryptosporidium infection in domestic cats from an urban area in Brazil

被引:1
|
作者
Neto, Luiz da Silveira [1 ]
Widmer, Giovanni [2 ]
Felix de Lima, Valeria Marcal [3 ]
Meireles, Marcelo Vasconcelos [3 ]
Inacio, Sandra Valeria [4 ]
Marques, Marcel Gambin [5 ]
Gregui Watanabe Marques, Ana Elisa [6 ]
Shigaki de Matos, Lucas Vinicius [7 ]
da Cruz Panegossi, Mariele Fernanda [8 ]
Saraiva Bresciani, Katia Denise [3 ]
机构
[1] Univ Fed Tocantins, Curso Grad Engn Bioproc & Biotecnol, Gurupi, TO, Brazil
[2] Tufts Univ, Cummings Sch Vet Med, North Grafton, MA 01536 USA
[3] Univ Estadual Paulista, Curso Grad Med Vet, UNESP, Aracatuba, SP, Brazil
[4] UNESP, Aracatuba, SP, Brazil
[5] UNIFIO, Curso Grad Med Vet, Ctr Univ Fac Integradas Ourinhos, Ourinhos, SP, Brazil
[6] UNESP, Programa Posgrad Ciencia Anim, Aracatuba, SP, Brazil
[7] Ipanema Ind Prod Vet, Pesquisa & Desenvolvimento, Aracoiaba Da Serra, SP, Brazil
[8] Ctr Univ Max Planck, Curso Med Vet, Indaiatuba, SP, Brazil
来源
SEMINA-CIENCIAS AGRARIAS | 2020年 / 41卷 / 06期
基金
巴西圣保罗研究基金会;
关键词
Prevalence; Public Health; Epidemiology; Cryptosporidiosis; Feline; GASTROINTESTINAL PARASITES; MOLECULAR CHARACTERIZATION; FECAL SAMPLES; RISK-FACTORS; PHYLOGENETIC ANALYSIS; GIARDIA-DUODENALIS; PARVUM OOCYSTS; SPP; DOGS; ANIMALS;
D O I
10.5433/1679-0359.2020v41n6p2677
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
We investigated the occurrence of Cryptosporidium oocysts shedding by domestic cats in an urban setting. The calculation of minimum sample size was based on an estimated prevalence of 10%, 5% absolute sampling error and a 5% significance level, resulting in 138 cats. A total of 612 owners of 2,290 cats had to be contacted for achieving the minimal sample size. In the end, only 55 owners accepted to participate in this investigation. Stool samples collected from 138 dogs were examined by microscopy using modified Kinyoun acid-fast staining, capture ELISA and nested-PCR followed by sequencing. Samples were considered positive when Cryptosporidium were detected by at least two diagnostic methods. Thirteen samples were positive (9.4%; 95% CI: 4.5 - 14.3). Cryptosporidium amplicons from seven out of the 13 samples were successfully sequenced and shared 99% genetic similarity to Cryptosporidium felis, GenBank access AF112575.1 was found. We concluded that Cryptosporidium infection is common in domestic cats from urban area and veterinary practitioners should guide cat owners to adopt preventive measures against the parasite to reduce the chance of infection in cats and householders.
引用
收藏
页码:2677 / 2685
页数:9
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