Human Umbilical Cord Blood Serum: Effective Substitute of Fetal Bovine Serum for Culturing of Human Multipotent Mesenchymal Stromal Cells

被引：8
|
作者：
Romanov, Yu. A. [1 ,3 ]
Balashova, E. E. [1 ,3 ]
Volgina, N. E. [2 ]
Kabaeva, N. V. [1 ]
Dugina, T. N. [3 ]
Sukhikh, G. T. [2 ]
机构：
[1] Minist Hlth Russian Federat, Russian Cardiol Res & Prod Complex, Moscow, Russia
[2] Minist Hlth Russian Federat, VI Kulakov Res Ctr Obstet Gynecol & Perinat, Moscow, Russia
[3] CryoCtr Cord Blood Bank, Moscow, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2017年 / 162卷 / 04期
基金：
俄罗斯科学基金会;
关键词：
multipotent mesenchymal stromal cells; umbilical cord blood serum; proliferation; phenotype; differentiation; STEM-CELLS; ADIPOSE-TISSUE; BONE-MARROW; DIFFERENTIATION; EXPANSION;
D O I：
10.1007/s10517-017-3654-9
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Optimal conditions for culturing of multipotent mesenchymal stromal cells in the presence of pooled umbilical cord blood serum were determined. It was found that umbilical cord blood serum in a concentration range of 1-10% effectively supported high viability and proliferative activity of cells with unaltered phenotype and preserved multilineage differentiation capacity. The proposed approach allows avoiding the use of xenogenic animal sera for culturing of multipotent mesenchymal stromal cells and creates prerequisites for designing and manufacturing safe cellular and/or acellular products for medical purposes.
引用
收藏
页码：528 / 533
页数：6
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