Two-enzyme system for the synthesis of 1-lauroyl-rac-glycerophosphate (lysophosphatidic acid) and 1-lauroyl-dihydroxyacetonephosphate

被引：5

作者：

Virto, C ^{[1
]}

Adlercreutz, P ^{[1
]}

机构：

[1] Univ Lund, Ctr Chem & Chem Engn, Dept Biotechnol, S-22100 Lund, Sweden

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2000年 / 104卷 / 02期

关键词：

phospholipase D; phospholipase C; acyl-phosphatidylglycerol; acyl-phosphatidyldihydroxyacetone; acyl-rac-glycerophosphate; acyl-dihydroxyacetonephosphate;

D O I：

10.1016/S0009-3084(99)00123-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A combination of two enzymes, phospholipase D (PL D) and C (PL C), was investigated for the production of two lysophospholipids, 1-lauroyl-rac-glycerophosphate (1-LGP) and 1-lauroyl-dihydroxyacetonephosphate (1-LDHAP). The high transphosphatidylation ability of phospholipase D from Streptomyces sp. allowed the formation of 1-lauroyl-phosphatidylglycerol (1-LPG) and 1-lauroyl-phosphatidyldihydroxyacetone (1-LPDHA) from phosphatidylcholine (PC) and 1-monolauroyl-rac-glycerol (1-MLG) and 1-lauroyl-dihydroxyacetone (1-MDHA), respectively. A two-phase system, diethyl ether/water, was chosen for the convenience of the recovery of the water insoluble products. A similar two-phase system was used for hydrolysis of the complex phospholipids by phospholipase C form Bacillus cereus, which released both lysophospholipids. Only trace amounts of phosphatidic acid (PA) were detected showing that the enzyme is highly selective for the release of the diacylglycerol and 1-lauroyl-rac-glycerophosphate and 1-lauroyl-dihydroxyacetonephosphate. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：175 / 184

页数：10

共 9 条

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