Removal of T4 lysozyme from silicon oxide surfaces by sodium dodecyl sulfate: A comparison between wild type protein and a mutant with lower thermal stability
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作者:
Wahlgren, M
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机构:Department of Food Technology, University of Lund, 221 00 Lund
Wahlgren, M
Arnebrant, T
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机构:Department of Food Technology, University of Lund, 221 00 Lund
Arnebrant, T
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[1] Department of Food Technology, University of Lund, 221 00 Lund
The adsorption and surfactant induced removal of T4 lysozyme was studied by in situ ellipsometry. Two proteins were investigated, wild type T4 lysozyme and a mutant protein where isoleucine 3 had been replaced by tryptophan (tryptophan mutant). The amount adsorbed is higher for the wild type protein than for the tryptophan mutant at the protein concentration employed (0.2 mg/mL). Furthermore, the adsorption kinetics differ between the two proteins, and the tryptophan mutant initially seems to adsorb at a somewhat slower rate than wild type protein, but the adsorbed amount levels off faster. Sodium dodecyl sulfate removes adsorbed proteins of both types from the silicon oxide surface, provided the surfactant concentration is high enough. The surfactant concentration needed to start removal was found to be well below cmc (3-11% of cmc in solution). The effect of ionic strength on this critical concentration follows the trends expected for surfactant aggregation and is not what would be expected for electrostatic interaction between protein and oppositely charged surfactant. This means that the removal starts at a lower surfactant concentration when the ionic strength is increased. Thus, the removal is thought to start at the concentration where the surfactant associates cooperatively to the adsorbed protein. The critical concentration for protein removal is higher, and the rate of protein removal is lower, at discrete surfactant concentration for wild type protein than for tryptophan mutant. This indicates that the onset of cooperative binding occurs at lower concentration for the tryptophan mutant, which is believed to be due to its larger changes in the conformation upon adsorption. These conformational changes might facilitate surfactant binding, for example, by exposing hydrophobic groups.
机构:
Japan Atom Energy Agcy, Quantum Beam Sci Ctr, Tokai, Ibaraki 3191195, Japan
Univ Hyogo, Dept Picobiol, 3-2-1 Koto, Kamigori Ako, Hyogo 6781297, JapanJapan Atom Energy Agcy, Quantum Beam Sci Ctr, Tokai, Ibaraki 3191195, Japan
Hiromoto, Takeshi
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Meilleur, Flora
Shimizu, Rumi
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Natl Inst Quantum & Radiol Sci & Technol, Quantum Beam Sci Res Directorate, 2-4 Shirakata, Tokai, Ibaraki 3191106, JapanJapan Atom Energy Agcy, Quantum Beam Sci Ctr, Tokai, Ibaraki 3191195, Japan
Shimizu, Rumi
Shibazaki, Chie
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Natl Inst Quantum & Radiol Sci & Technol, Quantum Beam Sci Res Directorate, 2-4 Shirakata, Tokai, Ibaraki 3191106, JapanJapan Atom Energy Agcy, Quantum Beam Sci Ctr, Tokai, Ibaraki 3191195, Japan
Shibazaki, Chie
Adachi, Motoyasu
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Natl Inst Quantum & Radiol Sci & Technol, Quantum Beam Sci Res Directorate, 2-4 Shirakata, Tokai, Ibaraki 3191106, JapanJapan Atom Energy Agcy, Quantum Beam Sci Ctr, Tokai, Ibaraki 3191195, Japan
Adachi, Motoyasu
Tamada, Taro
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Natl Inst Quantum & Radiol Sci & Technol, Quantum Beam Sci Res Directorate, 2-4 Shirakata, Tokai, Ibaraki 3191106, JapanJapan Atom Energy Agcy, Quantum Beam Sci Ctr, Tokai, Ibaraki 3191195, Japan
Tamada, Taro
Kuroki, Ryota
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Japan Atom Energy Agcy, Quantum Beam Sci Ctr, Tokai, Ibaraki 3191195, JapanJapan Atom Energy Agcy, Quantum Beam Sci Ctr, Tokai, Ibaraki 3191195, Japan